By H. Van Cauwenberge, P. Franchimont, P. Alexander and Z. M. Bacq (Auth.)
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Extra resources for Assay of Protein and Polypeptide Hormones
Example text
Grumbach and Kaplan (1962) have suggested heating the serum at 60°C for 40 minutes to eliminate the non-specific factors, on the theory that they are thermolabile. In actual fact, this approach does not completely resolve the problem of non-specific inhibition of hemagglutination. The sensitivity of the hemagglutination inhibition technique permits the evalution of quantitities of growth hormone on the order of 15-20 ng/ml but the precision is poor. CHnical utilization of this technique for assaying serum STH is limited principally by the presence of non-specific factors in the serum which cause inhibition of the hemagglutination.
10). This may be induced by insuUn (Roth et al, 1963, 1964; Hunter and Greenwood, 1964) or by tolbutamide (Hunter and Greenwood, 1964). However, hypo- and hyperglycemia as such do not appear to be the physiological stimuh involved. Roth et al (1963, 1964) showed that the administration of 2-deoxy-D-glucose, which inhibits intracellular utiliza tion of glucose and thus induces hyperglycemia, strongly stimulates the secretion of STH. The true stimulus thus appears to be the reduction in the amount of glucose that can be utiHzed by the cells rather than the blood glucose level itself.
And ALEXANDER, F . / . Clin. , 1 9 5 9 , 1 9 , 743. DOMÍNGUEZ, J. M. and PEARSON, O . H . / . Clin. , 1962, 2 2 , 856. ENGELS, G . and MORGALIN, S . Arch. Intern. , 1942, 7 0 , 236. FAIMAN, C . Nature {London), 1965, 2 0 5 , 8. FINK, C , JASIN, H . and Zur, Μ . Amer. J. Dis. , 1962, 1 0 4 , 562. FITSCHEN, W . Immunology, 1964, 7 , 307. FRANCHIMONT, P . Etude du dosage de certaines hormones hypophysaires. Arscia, Bruxelles /Maloine, Paris, 1964. FRANCHIMONT, P. Ann. Endocr. {Paris), 1965, 2 6 , 346.