By Arthur I. Spriggs DM, FRCP, FRCPath, Michael M. Boddington MSc, FRCPath (auth.)
It is was hoping that this booklet may well serve reasons. felt that the ensuing elevate in dimension and price of First it really is meant to be used on the bench. to aid resolve the publication wouldn't be justified. a few of the difficulties of mobilephone id which the fabric used to be amassed over a long time and puzzle not just rookies. yet even skilled we won't separately recognize the nice contri observers within the box of cytological prognosis. Sec bution made by way of technical employees of the medical cytology ondly we now have reviewed the main major prior laboratories who assisted the 2 authors to organize courses. relatively these of the previous two decades. and stain the sl ides. lots of the electron micrographs so that it will offer a resource of reference. have been made via or with the help of the overdue Denis Cytological analysis shouldn't be remoted from W. Jerrome. and we're additionally thankful to Dr David histopathology. and plenty of readers will remorse the shortcoming Ferguson for additional aid and recommendation with those. all the immunocytochemical stains have been performed by means of Dr of cyto-histological correlation in our selection of images. Cells centrifuged out of suspension in Anna Ghosh. and Drs David Mason and Kevin Gatter pathological fluids endure little or no resemblance to kindly commented on that a part of the manuscript. their opposite numbers in tissue sections.
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Additional info for Atlas of Serous Fluid Cytopathology: A Guide to the Cells of Pleural, Pericardial, Peritoneal and Hydrocele Fluids
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Probably resulting from an old tuberculous empyema. Abundant cholesterol crystals gave a shimmering effect to the fluid. Wet preparation. phase contrast. x 250 19b Male aged 64. Cholesterol pleural effusion. probably resulting from unabsorbed post - pneumonic effusion. In Giemsa smears the crystals are seen unstained on a mauve background . MGG x 200 ,• 19d Same ca se as Plate 19c. Near centre an LE cell is seen - a darkly stained hyaline inclusion occupies the cytoplasm of a neutrophil. in which the lobes are not separately distinguishable.
Most of the histochemical staining methods used in histopathology are applicable to cytological smears, but modification is usually necessary and interpretation needs special experience for any given test. While not belittling their value for research, it has to be said that very few have been found useful for routine diagnosis. The demonstration of intracellular epithelial mucin is valuable in revealing mucus-secreting carcinoma cells, because mesothelial cells do not have this capacity. The periodic acid-Schiff routine (PAS), with and without amylase or diastase digestion, is the most satisfactory method (see page 129).